Reagents and instruments for immunology, cell biology and molecular biology.

IgM (TRITC)

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Cat# I1905-02D-2mg

Size : 2mg

Brand : US Biological

I1905-02D IgM (TRITC)

Clone Type

Polyclonal

Host

goat

Source

mouse

Isotype

IgG

Grade

Affinity Purified

Applications

E IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)||Absorption Wavelength: 550 nm||Emission Wavelength: 570 nm||Fluorochrome/Protein Ratio: 3.3 moles TRITC per mole of Goat IgG||Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. R||ecommended Dilution: Optimal dilutions to be determined by the researcher||Recommended Dilution:|Immunofluorescence Microscopy: 1:500-1:2500|Other applications have not been tested.||Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: mouse|Concentration: ~2mg/ml (UV280)|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide, 10mg/ml IgG and protease-free BSA. Labeled with TRITC.|Purity: Purified by immunoafﬁnity chromatography.|Immunogen: Mouse IgM mu chain|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against Mouse IgG or Mouse IgA. Speciﬁcity was conﬁrmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Mouse IgM mu chain

Form

Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide, 10mg/ml IgG and protease-free BSA. Labeled with TRITC.

Purity

Purified by immunoafﬁnity chromatography.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against Mouse IgG or Mouse IgA. Speciﬁcity was conﬁrmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.