Leupeptin (hemisulfate) [103476-89-7]
Cat# HY-18234A-5mg
Size : 5mg
Brand : MedChemExpress
Leupeptin hemisulfate is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin hemisulfate potently inhibits serine, cysteine and threonine proteases. Leupeptin hemisulfate inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity.
For research use only. We do not sell to patients.
Leupeptin hemisulfate Chemical Structure
CAS No. : 103476-89-7
This product is a controlled substance and not for sale in your territory.
Based on 29 publication(s) in Google Scholar
Other Forms of Leupeptin hemisulfate:
- Leupeptin Get quote
- Leupeptin Ac-LL Get quote
- Leupeptin Ac-LL hydrochloride Get quote
Description |
Leupeptin hemisulfate is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin hemisulfate potently inhibits serine, cysteine and threonine proteases. Leupeptin hemisulfate inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3]. |
IC50 & Target |
Cathepsin B, Cathepsin H, Cathepsin L, Ser/Thr Protease, Mpro[1][2][3]. |
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In Vitro |
Leupeptin hemisulfate (0.06-200 µM; 72 h) significantly decreases copy numbers of SARS-CoV-2 viral RNA (vRNA) in Vero cells[1] MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. RT-PCR[1]
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In Vivo |
Leupeptin hemisulfate (0, 9, 18, 36 mg/kg; i.p.; single) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Molecular Weight |
475.59 |
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Formula |
C20H38N6O4.1/2H2SO4 |
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CAS No. |
103476-89-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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Sequence Shortening |
Ac-LLR-CHO |
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SMILES |
O=C[C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)CCCNC(N)=N.[0.5H2SO4] |
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Structure Classification |
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Initial Source |
Microbial Metabolite |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
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Solvent & Solubility |
In Vitro:
H2O : 83.33 mg/mL (175.21 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation |
Purity: 98.0% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O | 1 mM | 2.1027 mL | 10.5133 mL | 21.0265 mL | 52.5663 mL |
5 mM | 0.4205 mL | 2.1027 mL | 4.2053 mL | 10.5133 mL | |
10 mM | 0.2103 mL | 1.0513 mL | 2.1027 mL | 5.2566 mL | |
15 mM | 0.1402 mL | 0.7009 mL | 1.4018 mL | 3.5044 mL | |
20 mM | 0.1051 mL | 0.5257 mL | 1.0513 mL | 2.6283 mL | |
25 mM | 0.0841 mL | 0.4205 mL | 0.8411 mL | 2.1027 mL | |
30 mM | 0.0701 mL | 0.3504 mL | 0.7009 mL | 1.7522 mL | |
40 mM | 0.0526 mL | 0.2628 mL | 0.5257 mL | 1.3142 mL | |
50 mM | 0.0421 mL | 0.2103 mL | 0.4205 mL | 1.0513 mL | |
60 mM | 0.0350 mL | 0.1752 mL | 0.3504 mL | 0.8761 mL | |
80 mM | 0.0263 mL | 0.1314 mL | 0.2628 mL | 0.6571 mL | |
100 mM | 0.0210 mL | 0.1051 mL | 0.2103 mL | 0.5257 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.