Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG1 Hinge secondary antibody, clone 4E3 (Alkaline Phosphatase).
- Specifications
Product Description
Mouse monoclonal antibody raised against human IgG1 Hinge.
Immunogen
Unknown.
Host
Mouse
Theoretical MW (kDa)
146
Reactivity
Human
Specificity
The antibody reacts with human IgG1 hinge.
Form
Liquid
Conjugation
Alkaline Phosphatase
Purification
Precipitation and/or chromatography
Purity
>= 90% purity by HPLC and/or SDS-PAGE
Isotype
IgG1, kappa
Recommend Usage
ELISA (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris, 1 mM MgCl2, pH 8.0 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG1 Hinge secondary antibody, clone 4E3 (Alkaline Phosphatase). - Gene Info — IGHG1
- Interactomes
- Diseases