Mouse Casp8 (Caspase-8) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Casp8
Gene ID:	12370
Uniprot ID:	CASP8_MOUSE
Specificity:	This kit recognizes Mouse CASP8 in samples. No significant cross-reactivity or interference between Mouse CASP8 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. (Microbial infection) Proteolytically cleaved by the cowpox virus CRMA death inhibitory protein. Phosphorylation on Ser-389 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
Function	Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor TNFRSF6/FAS mediated or TNFRSF1A. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-325', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response. Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis. Initiates pyroptosis following inactivation of MAP3K7/TAK1. Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production. May participate in the Granzyme B (GZMB) cell death pathways. Cleaves PARP1 and PARP2.
Protein Name	Caspase-8 Casp-8 Cleaved Into - Caspase-8 Subunit P18 - Caspase-8 Subunit P10
Database Links	Reactome: R-MMU-111465 Reactome: -MMU-140534 Reactome: -MMU-168638 Reactome: -MMU-2562578 Reactome: -MMU-264870 Reactome: -MMU-3371378 Reactome: -MMU-5218900 Reactome: -MMU-5357905 Reactome: -MMU-5660668 Reactome: -MMU-5675482 Reactome: -MMU-69416 Reactome: -MMU-75108 Reactome: -MMU-75153 Reactome: -MMU-75157 Reactome: -MMU-75158 Reactome: -MMU-9758274
Cellular Localisation	Cytoplasm Nucleus Translocates Into The Nucleus During Apoptosis
Alternative ELISA Names	Caspase-8 ELISA kit Casp-8 Cleaved Into - Caspase-8 Subunit P18 - Caspase-8 Subunit P10 ELISA kit Casp8 ELISA kit
Specificity	This kit recognizes Mouse CASP8 in samples. No significant cross-reactivity or interference between Mouse CASP8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-99	91
EDTA plasma (n=8)	86-99	93
Cell culture media (n=8)	91-105	98

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.54	1.36	4.58	0.52	1.33	4.37
Standard deviation	0.03	0.07	0.24	0.03	0.07	0.18
CV (%)	5.56	5.15	5.24	5.77	5.26	4.12

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance