Quantity | 50 µg |
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Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG1 |
Conjugate | Unconjugated |
Application | IHC, WB |
Purification | Affinity chromatography |
Reactivity | Human, Mouse, Rat |
Storage before reconstitution | 2-8°C until indicated expiry date |
Storage after reconstitution | Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Clone | Dsc1-U100 |
Immunogen | Synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC) |
Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
UniprotID | |
Synonym | Desmocollin-1, DSC1, Cadherin family member 1, Desmosomal glycoprotein 2/3, DG2/DG3, CDHF1 |
Tested applications | Tested dilutions |
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Immunohistochemistry (IHC) - frozen | 1:50-1:100 (preincubation (directly after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended; without detergent pretreatment a cytoplasmic component might cross-react) |
Immunohistochemistry (IHC) - paraffin | 1:50-1:100 (microwave treatment recommended; a slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step) |
Western Blot (WB) | Assay dependent |
Mouse monoclonal antibody localizes Dsc1 present in
Reacts with 111 kDa (form "a") and 103 kDa (form "b") polypeptides (desmocollin 1 splice forms, both at pI 5.2) from human epidermal desmosomes.
Reactivity on cultured cell lines: positive on primary keratinocytes, however, restricted to local piles of differentiated cells. All simple epithelia are negative.