NHEK/SVTERT3-5 cells show an extended in vitro life span and express typical functions of skin keratinocytes as they can be differentiated towards 3D skin equivalents in an air-liquid interface. Thus, the cell line can be used as standardized in vitro model to study processes such as wound healing or to investigate the uptake of certain drugs. Additionally, due to its unique characteristics, the cell line allows studying chronic intoxication as demonstrated using arsenic.
General information
Cat#: CLHT-011-0026-5
Morphology and 3D differentiation
NHEK/SVTERT3-5 cells show the typical cobblestone epithelial morphology of keratinocytes and can be differentiated towards a stratified skin equivalent in an air-liquid interface.
In vitro propagation
Keratinocyte growth medium KGM Gold + G418
KBM Gold basal medium (Lonza, Cat# 00192151)
Components of KGM Gold SingleQuot Kit (Lonza, Cat# 00192152: BPE, hEGF, insulin, hydrocortisone, transferrin, epinephrine)
Additional material & reagents
Phosphate buffered saline (PBS) (Sigma, Cat# D8537)
Trypsin inhibitor (Gibco, Cat# R007100)
0,05 % Trypsin-EDTA (Gibco, Cat#25300-054)
EASY cell strainer (Greiner, Cat# 542000)
Passaging of cells
For detachment of the cells remove and discard the culture medium and wash the cells once with PBS (about 160 µl/cm²). Remove PBS completely.