Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
Cat# PAA919Hu01-20ul
Size : 20ul
Brand : USCN
Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
LAD; GCSL; Dihydrolipoamide Dehydrogenase; E3 Component Of Pyruvate Dehydrogenase Complex; 2-Oxo-Glutarate Complex; Branched Chain Keto Acid Dehydrogenase Complex
- Product No.PAA919Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA919Hu01-Recombinant Dihydrolipoyl Dehydrogenase (DLD)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/mL
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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Packages (Simulation) -
Packages (Simulation)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human DLD Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human DLD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human DLD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) - FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
- Figure. Western Blot; Sample: Recombinant DLD, Human.
- Western Blot; Sample: Mouse Lung lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: DLD knockout HepG2 cell lysate;
Predicted MW: 54kd
Observed MW: 58kd
Primary Ab: 1µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human DLD Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
The antibody is a rabbit polyclonal antibody raised against DLD. It has been selected for its ability to recognize DLD in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Dihydrolipoyl Dehydrogenase (DLD)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.