ProteinExt® Mammalian Nuclear and Cytoplasmic Protein Extraction Kit
Cat# DE201-01
Size : 50rxns
Brand : TransGen Biotech
ManualProteinExt® Mammalian Nuclear and Cytoplasmic Protein Extraction Kit provides a fast and efficient method to extract nuclear and cytoplasmic proteins from mammalian cells and tissues. Native proteins can be obtained within 80 minutes without ultracentrifugation. The extracted proteins are suitable for a variety of downstream applications, including SDS-PAGE, Western blot, ELISA, enzyme-activity assays, immunoprecipitation and transcription factor activity analysis.
Storage
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×) at -20℃ for one year; Others at 2-8℃ for one year.
Shipping
Ice bag (4℃).
| Component | DE201-01 (50 rxns) |
| Cytoplasmic Protein Extraction Buffer I (CPEB I) | 75 ml |
| Cytoplasmic Protein Extraction Buffer II (CPEB II) | 3 ml |
| Nuclear Protein Extraction Buffer (NPEB) | 25 ml |
| ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×) | 1 ml |
Liu Y , Fang S , Li X , et al. Aspirin inhibits LPS-induced macrophage activation via the NF-κB pathway[J]. Sci Rep, 2017, 7(1):11549.
Xu X , Qimuge A , Wang H , et al. IRE1 alpha XBP1s branch of UPR links HIF1 alpha activation to mediate ANGII-dependent endothelial dysfunction under particulate matter (PM) 2.5 exposure[J]. SCIENTIFIC REPORTS, 2017, 7(1):13507.