PtX™ Mouse Anti-Interferon Gamma Recombinant Antibody
Mouse Anti-IFN-Gamma
Recombinant mouse monoclonal antibody against Interferon-Gamma cytokine. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.
100 µg | Catalogue Number: CBT_A0012
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Interferon-Gamma (IFN-γ) is a pleiotropic cytokine and the only member of the type II IFN family, which differs structurally and functionally from other types of IFNs. IFN-γ is a homodimer synthesized by non-covalent association of two 146 amino acid subunits in an antiparallel manner, forming a mature protein of approximately 33 kDa. Antigens, mitogens and cytokines, e.g. IL-12, IL-15 and IL-18, induce production of IFN-γ by T lymphocytes and natural killer cells. Once released, it acts as macrophage activation factor through binding of IFN-γ receptor I and II and subsequent activation of the JAK/STAT pathway. The inhibitory effects of IFN-γ on viral replication were originally reported in 1965 and its role in host response to microorganisms has been well established. IFN-γ also plays an important role in cancer immune surveillance, promoting tumor recognition and immunity.
Additional information
| Available Unit Size | 0.1mg, 1mg, 10mg, 100mg, 200mg |
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Key Benefits
This product is a recombinant monoclonal antibody that offers several benefits including the following:
- Batch-to-batch consistency.
- No animals or animal products are used in the development of this protein.
- BSA and Azide free.
For more information on plant-made, recombinant monoclonal antibodies click here.
Datasheet
| Product Name | PtX™ Mouse Anti-Interferon-Gamma Recombinant Antibody |
| Catalogue Number | CBT_A0012 |
| Expression Host | Nicotiana benthamiana plants |
| Clonality | Monoclonal, recombinant |
| Species and Isotype | Mouse IgG2a |
| Tag | None |
| Reporter Protein | None |
| Description | Recombinant mouse monoclonal antibody against IFN-γ cytokine, produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants. |
| Verified Applications | Western blot, ELISA. |
| Recommended dilutions | Western blots (1: 2 000 – 1: 10 000) ELISA (1: 1 000 – 1: 5 000) |
| Concentration | 1.0 mg/ml |
| Form | Liquid |
| Colour | Clear |
| Preparation | Ready to use |
| Storage | Short-term (up to one week): 2 – 8 °C Long term: Aliquot and store at – 20 °C Store immediately. Aliquot and avoid multiple freeze-thaw cycles. |
| Storage buffer | 0.1 M Phosphate Buffered Saline, pH 7.4. Preservative: none |
| Purification notes | This product was purified using Protein A affinity chromatography. |
| Purity | ≥ 90 % as determined by SDS-PAGE. |
| General notes | If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing . |
Images
Figure 1: ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of antibody added to 96 well plate coated with 0.3 ng/µl of IFN antigen.
Figure 2: Western blot analysis of PtX™ Mouse Anti-IFN-Gamma. Lanes 2–5: Varying amounts of IFN-γ were run on the SDS-PAGE. Separated bands were transferred to the membrane and PtX™ Mouse Anti-IFN- Gamma (1: 2 500) was used to detect the antigen. The two bands of IFN-Gamma were visualized following the addition of anti-rabbit secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 31 ng (Lane 5).
PtX™ | Plant-Based Expression
The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.
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