General Info
| Applications: | CLIA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 4.69pg/mL |
| Detection Limit: | 7.81~500pg/mL |
| Short Description: | This rat CTSD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | CtsdUniprot ID=CATD_RAT" |
| Specificity: | This kit recognizes Rat CTSD in samples. No significant cross-reactivity or interference between Rat CTSD and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Post Translational Modifications | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. |
| Function | Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. |
| Protein Name | Cathepsin D Cleaved Into - Cathepsin D 12 Kda Light Chain - Cathepsin D 9 Kda Light Chain - Cathepsin D 34 Kda Heavy Chain - Cathepsin D 30 Kda Heavy Chain |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular Space |
| Alternative CLIA Names | Cathepsin D Cleaved Into - Cathepsin D 12 Kda Light Chain - Cathepsin D 9 Kda Light Chain - Cathepsin D 34 Kda Heavy Chain - Cathepsin D 30 Kda Heavy Chain CLIA kitCtsd CLIA kit |
| Specificity | This kit recognizes Rat CTSD in samples. No significant cross-reactivity or interference between Rat CTSD and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-102 | 95 |
| EDTA plasma(n=8) | 85-100 | 92 |
| Cell culture media(n=8) | 84-98 | 90 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 23.43 | 58.78 | 171.87 | 24.29 | 56.58 | 185.13 |
| Standard deviation | 2.54 | 5.81 | 16.29 | 3.04 | 5.04 | 15.88 |
| CV (%) | 10.84 | 9.88 | 9.48 | 12.52 | 8.91 | 8.58 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance