Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.

• K_d: 1 uM
• λ_Ex/λ_Em (low or high [Ca²⁺])= 556/576 nm
• ε (556 nm) = 80,000 M^-1cm^-1
• Orange red solid soluble in DMSO and water (pH >6)
• Store at 4°C and protect from light, especially in solution
• C₄₀H₃₉N₄Na₃O₁₁
• MW: 945

 
 
BAPTA-based ion indicators like Rhod-2 have been shown to be fixable in situ by

EDC/EDAC (cat# 59002)

Cell Calcium 1997, 21(3), 175

As the indicator does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. The rate of efflux increases with temperature, and may vary between cell types, resulting in variable retention times of a few minutes to hours. Experiments using indicators in cells usually are performed within one or two hours of loading, but it may be possible to re-load cells with indicator if needed. The organic anion transporter inhibitor

Probenecid (#50027)