Uric Acid (UA) Colorimetric Assay Kit
Cat# E-BC-K016-M-500
Size : 500Assays
Brand : Elabscience
| Detection Principle | Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Uric acid content can be calculated by measuring the OD value at 690 nm. |
| Synonyms | UA |
| Sample Type | Serum,Plasma,Urine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (680-700 nm, optimum wavelength: 690 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.30 mg/L |
| Detection Range | 1.30-80 mg/L |
| Precision | inter-assay CV: 0.04 | intra-assay CV: 0.02 |
| Sample Volume | 25 μL |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1-2 |
| Rat serum | 1 |
| Human serum | 1 |
| Porcine serum | 1 |
| Dog serum | 1-2 |
| Human urine | 8-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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