Immunophenotyping & Lymphocyte Subset Characterization

Immunophenotyping & Lymphocyte Subset Characterization

Assays in whole blood, PBMCs and purified immune cell subsets

Immunophenotyping&Lymphocyte Subset Characterization

We offer assays in whole blood, PBMCs and purified immune cell subsets. Multicolor (up to 5 color) immunphenotyping and functional analysis of immune cells is an area of particularly strong experience at Aragen. In addition to standard flow cytometry, Aragen has the capability of high-throughput 4-color flow cytometry (with the IntelliCyt iQue system) for many single and multiplexed assays. As an example of a multi-panel, multicolor immunophenotyping project, the panels of antibodies shown below were used to stain whole blood samples to determine the effect of test antibodies on the frequency and absolute number of specific cell subsets in peripheral blood.

Cell types
   
   
Markers
   
   
T, B, NK Cells
CD45
CD3
CD4/8
CD19
CD56
Monocytes
CD45
CD14
CD16
CD11b
   
Granulocytes
CD45
CD15
CD33
CD66b
  
Treg cells
CD45
CD4
CD25
CD127
FoxP3
CD
Lin
HLA-DR
CD123
CD11c
   

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