Pyranose Oxidase antibody (Peroxidase)
Cat# orb344269-100ug
Size : 100ug
Brand : Biorbyt
Pyranose Oxidase antibody (Peroxidase)
Catalog Number: orb344269
| Catalog Number | orb344269 |
|---|---|
| Category | Antibodies |
| Description | Pyranose Oxidase antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Pyranose Oxidase [E.coli] |
| Concentration | 1 mg/mL |
| Dilution range | ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | Pyranose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli]. Cross reactivity against Pyranose Oxidase from other sources is unknown. |
| Conjugation | HRP |
| UniProt ID | P79076 |
| NCBI | Q5G234.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | goat anti-Pyranose Oxidase Antibody, HRP Conjugate |
| Note | For research use only |
| Application notes | Anti-Pyranose Oxidase has been assayed against 1.0 ug of Pyranose Oxidase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti Pyranose Oxidase antibody was used to detect pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/orb348637 1 hr RT).