The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 19(MMP19). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 19(MMP19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 19(MMP19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 19(MMP19) in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity
Hamster
Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL
Protein Length
3.5h
Alternative names
RASI-1, MMP18, Occasionally Referred To As Stromel
Note
For research use only
Sample Types
Tissue homogenates, cell lysates and other biological fluids.
