Notch1 antibody
Cat# orb150328-100ug
Size : 100ug
Brand : Biorbyt
Notch1 antibody
Catalog Number: orb150328
| Catalog Number | orb150328 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal to Notch1 (ATTO-488). Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | S253-32 |
| Tested applications | ICC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 |
| Immunogen | Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000) |
| Conjugation | ATTO-488 |
| MW | 270kDa |
| Target | Notch1 |
| Entrez | 18128 |
| UniProt ID | Q01705 |
| NCBI | NP_032740.3 |
| Storage | Conjugated antibodies should be stored at 4°C |
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Alternative names | Neurogenic locus notch homolog protein 1 antibody, |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |