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Chemiluminescent Western Blot Kit

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Cat# orb342275

Size : 1Kit

Brand : Biorbyt


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    Chemiluminescent Western Blot Kit

    Catalog Number: orb342275

    Catalog Numberorb342275
    CategoryAssays and Kits
    DescriptionChemiluminescent Western Blot Kit
    Tested applicationsWB
    ReactivityE. coli
    ImmunogenAnti-Host Cell Protein Antibody (p/n 100-401-J07) was prepared blending antibodies that have been separately developed by repeated immunizations in rabbit with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli (BL21).
    ConcentrationN/A
    Dilution rangeWB: User Optimized by Kit Protocol
    Form/Appearancen/a
    PurityThis Chemiluminescent Western Blot Kit allows for the detection of E.coli host cell proteins present in samples provided by the user. Included in the kit along with Rabbit Anti-Host Cell Protein antibody is a positive protein control, Goat Anti-Rabbit IgG HRP Antibody, and Blocking Buffer.
    ConjugationUnconjugated
    StorageSee kit insert for complete instructions.
    Alternative namesWestern Blotting Kit, Chemiluminescent Kit, Peroxi
    NoteFor research use only
    Application notesUse Biorbyt’ Chemiluminescent Western Blot Kit for detection of E.coli Host Cell Proteins. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-Host Cell Proteins Antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with Femtomax, a specialized formulation of luminol which is an extremely sensitive, non-radioactive substrate that emits light (chemiluminescence) and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Femtomax reagent (FEMTOMAX-020 or FEMTOMAX-110) is not included with this kit and must be purchased separately.
    Expiration DatePlease enquire.
    Chemiluminescent Western Blot Kit

    2D Western Blot of anti-E.coli HCP antibody. Load: 35 µg Total HCP. pH: 5-8. Primary antibody: Rabbit anti-HCP antibody at 1:200 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 for 1 hour at RT.

    Chemiluminescent Western Blot Kit

    Western Blot of Rabbit anti-HCP antibody. Lane 1: Molecular Weight. Lane 2: Total HCP. Load: 10 µg per lane. Primary antibody: Rabbit anti-HCP cocktail at 1:50 for overnight at 4°C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10000 for 30 min at RT. Block: orb348637 for 1 hour at RT.

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