Blocking Buffer For Fluorescent Western Blotting

Cat# orb348637-500ml

Size : 500ml

Brand : Biorbyt


    Blocking Buffer For Fluorescent Western Blotting

    Catalog Number: orb348637

    Catalog Numberorb348637
    CategoryProteins
    DescriptionBlocking Buffer For Fluorescent Western Blotting
    Tested applicationsWB
    Concentration1X
    Dilution rangeWB: User Defined
    Form/AppearanceLiquid (sterile filtered)
    PurityBlocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
    ConjugationUnconjugated
    Hazard InformationNon-Toxic
    StorageStore Blocking Buffer at 4° C prior to opening. DO NOT FREEZE.
    Buffer/PreservativesThimerosal is added as an antimicrobial agent.. See application note.
    Alternative namesMultiplex Blocking Buffer, Fluorescent Blocking Bu
    NoteFor research use only
    Application notesFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications. Antibody conjugates prepared with IRDye 800 and IRDye 700DX (Licor), Cy2, Cy3, Cy3.5, Cy5 and Cy5.5 (GE Healthcare), DyLight405, DyLight 549, DyLight 649, DyLight 680, and DyLight 800 (Thermo Fisher/Pierce) and Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 647 and Alexa Fluor 680 (Invitrogen/Molecular Probes) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with this product can be dried and are very stable. Membranes that are stored protected from light can be re-washed and/or rescanned.
    Expiration Date6 months from date of receipt.
    Blocking Buffer For Fluorescent Western Blotting

    Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIP® Peptide Microarrays. The PEPperCHIP® Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Biorbyt Blocking Buffer [p/n orb348637] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti-Human IgG (H+L) DyLight™ 680 Antibody and a control anti-HA (12CA5)-DyLight™ 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.

    Blocking Buffer For Fluorescent Western Blotting

    Selected sections of the PEPperCHIP® Peptide Microarrays after assay with different blocking reagents. The microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Biorbyt Blocking Buffer [p/n orb348637] (D), respectively. A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat Anti-Human IgG (H+L) DyLight™ 680 Antibody. Red spots = sample responses and polio control peptides, green spots = HA control peptides. The underlying binding motifs of the respective sections are indicated on the left.