Blocking Buffer For Fluorescent Western Blotting
Cat# orb348637-500ml
Size : 500ml
Brand : Biorbyt
Edmiston, Elizabeth et al. Identification of the antigenic epitopes of maternal autoantibodies in autism spectrum disorders Brain Behav Immun, 69, 399-407 (2018)
Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIP® Peptide Microarrays. The PEPperCHIP® Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Biorbyt Blocking Buffer [p/n orb348637] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti-Human IgG (H+L) DyLight™ 680 Antibody and a control anti-HA (12CA5)-DyLight™ 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.
Selected sections of the PEPperCHIP® Peptide Microarrays after assay with different blocking reagents. The microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Biorbyt Blocking Buffer [p/n orb348637] (D), respectively. A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat Anti-Human IgG (H+L) DyLight™ 680 Antibody. Red spots = sample responses and polio control peptides, green spots = HA control peptides. The underlying binding motifs of the respective sections are indicated on the left.