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Chemiluminescent Western Blot Kit

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Cat# orb342274

Size : 1Kit

Brand : Biorbyt


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Chemiluminescent Western Blot Kit

Catalog Number: orb342274

Catalog Numberorb342274
CategoryAssays and Kits
DescriptionChemiluminescent Western Blot Kit
Tested applicationsWB
Concentrationn/a
Dilution rangeWB: User Optimized
Form/Appearancen/a
PurityThis Chemiluminescent Western Blot Kit allows for the detection of FLAG -tagged recombinant protein present in cell lysates provided by the user.
ConjugationUnconjugated
Hazard InformationNon-Toxic
StorageSee kit insert for complete instructions.
Buffer/PreservativesWash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
Alternative namesFlag Western Blot, Western Blotting Kit, Chemilumi
NoteFor research use only
Application notesUse Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western Blotting for detection of FLAG -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Expiration DatePlease enquire.
Chemiluminescent Western Blot Kit

Flow Diagram for Chemiluminescent Western Blot Procedure

Chemiluminescent Western Blot Kit

Western Blot. Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western blot was used to detect known amounts of bacterial alkaline phosphatase (BAP) protein containing the FLAG epitope tag. N-terminal linked and C-terminal linked BAP were diluted into a HeLa

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