DPP4 Antibody
Cat# orb1259899-50ul
Size : 50ul
Brand : Biorbyt
Item 1 of 4
Item 1 of 4
DPP4 Antibody
Catalog Number: orb1259899
| Catalog Number | orb1259899 |
|---|---|
| Category | Antibodies |
| Description | DPP4 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 29-280 of human DPP4 (NP_001926.2). |
| Concentration | batch dependent |
| Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 110kDa |
| Target | DPP4 |
| UniProt ID | P27487 |
| Storage | Store at -20°C. Avoid freeze / thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Alternative names | Dipeptidyl peptidase 4, ADABP, Adenosine deaminase |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DPP4 antibody (orb1259899) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunofluorescence analysis of C6 cells using DPP4 antibody (orb1259899) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using DPP4 antibody (orb1259899) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using DPP4 antibody (orb1259899) at dilution of 1:100. Blue: DAPI for nuclear staining.