Proteinase K - Solid
Cat# orb532710-200mg
Size : 200mg
Brand : Biorbyt
Proteinase K - Solid
Catalog Number: orb532710
| Catalog Number | orb532710 |
|---|---|
| Category | Tools |
| Description | Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5). Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF. In presence of 0.5-1% SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids. |
| CAS Number | 39450-01-6 |
| Form/Appearance | powder; Color: white |
| Purity | free of RNases, DNases and Exonucleases |
| MW | Theoretical MW: 28.9 kDa |
| Biological Activity | Specific activity: > 30 units/mg, DNase activity: not detectable, RNase activity: not detectable |
| Storage | store at -20 °C |
| Note | For research use only |
| Application notes | Digestion of proteins during DNA and RNA preparation. |
| Expiration Date | 6 months from date of receipt. |