Glycolysis
Glucose is used during the first step of glycolysis to keep its concentration at a low level and to promote a continuous transport of glucose into the cells through the plasma membrane transporters.
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Phosphorylation of Glucose by hexokinase forms Glucose-6-phosphate with the use of ATP.
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Glucose-6-phosphate is a key sugar intermediate for glucose to get into cells and then enter either metabolic pathways or storage.
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Phosphoglucose isomerase rearranged Glucose-6-phosphate into Fructose-6-phosphate via isomerization.
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Please find our assay kits for Fructose-6-phosphate.
The formation of Fructose-1,6-bisphophate catalyzed by Phosphofructokinase (PFK), coupled to the hydrolysis of ATP, is irreversible and a rate-limiting step.
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Fructose-1,6-bisphosphate lies within the glycolysis metabolic pathway.
Fructose-1,6-bisphosphate is split into two triose sugars, dihydroxyacetone phosphate, a ketose, and glyceraldehyde-3-phosphate, an aldose.
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Glyceraldehyde 3-phosphate is a chemical compound that occurs as an intermediate in several central metabolic pathways.
The aldehyde group of the Glyceraldehyde-3-phosphate is oxidised and an inorganic phosphate is added to them by the glyceraldehyde phosphate dehydrogenase, forming the 1,3 bisphophoglycerate.
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1,3-bisphosphoglycerate is a metabolic intermediate in glycolysis and Calvin cycle.
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3-phosphglycerate is a metabolic intermediate in both glycolysis and the Calvin cycle.
Phosphoglycerate mutase isomerises 3-phosphoglycerate into 2-phosphoglycerate.
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Enolase forms phosphoenolpyruvate from 2-phosphoglycerate.
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A final step catalyzed by pyruvate kinase forms a molecule of pyruvate and a molecule of ATP.
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